EMBL Genomics Core Facility
Sample Submission
User Information
Username
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Group Name
*
Email
*
Sequencer Settings
Sequencing Technology
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Illumina
PacBio
Illumina sequencing modes
MiSeq i100 5M 1 x 300bp Single-end
MiSeq i100 5M 2 x 150bp Paired-end
MiSeq i100 5M 1 x 600bp Single-end
MiSeq i100 5M 2 x 300bp Paired-end
MiSeq i100 25M 1 x 100bp Single-end
MiSeq i100 25M 2 x 50bp Paired-end
MiSeq i100 25M 1 x 300bp Single-end
MiSeq i100 25M 2 x 150bp Paired-end
MiSeq i100 25M 1 x 600bp Single-end
MiSeq i100 25M 2 x 300bp Paired-end
NextSeq2000 P1 100bp Single-end
NextSeq2000 P1 2 x 50bp Paired-end
NextSeq2000 P1 300bp Single-end
NextSeq2000 P1 2 x 150bp Paired-end
NextSeq2000 P1 600bp Single-end
NextSeq2000 P1 2 x 300bp Paired-end
NextSeq2000 P2 100bp Single-end
NextSeq2000 P2 2 x 50bp Paired-end
NextSeq2000 P2 200bp Single-end
NextSeq2000 P2 2 x 100bp Paired-end
NextSeq2000 P2 300bp Single-end
NextSeq2000 P2 2 x 150bp Paired-end
NextSeq2000 P2 600bp Single-end (300 M clusters!)
NextSeq2000 P2 2 x 300bp Paired-end (300 M clusters!)
NextSeq2000 P3 100bp Single-end
NextSeq2000 P3 2 x 50bp Paired-end
NextSeq2000 P3 200bp Single-end
NextSeq2000 P3 2 x 100bp Paired-end
NextSeq2000 P3 300bp Single-end
NextSeq2000 P3 2 x 150bp Paired-end
NextSeq2000 P4 50bp Single-end
NextSeq2000 P4 2 x 36bp Paired-end
NextSeq2000 P4 100bp Single-end
NextSeq2000 P4 2 x 50bp Paired-end
NextSeq2000 P4 200bp Single-end
NextSeq2000 P4 2 x 100bp Paired-end
NextSeq2000 P4 300bp Single-end
NextSeq2000 P4 2 x 150bp Paired-end
NovaSeq X 1.5B 1 x 100bp Single-end
NovaSeq X 1.5B 2 x 50bp Paired-end
NovaSeq X 1.5B 1 x 200bp Single-end
NovaSeq X 1.5B 2 x 100bp Paired-end
NovaSeq X 1.5B 300bp Single-end
NovaSeq X 1.5B 2 x 150bp Paired-end
NovaSeq X 10B 1 x 100bp Single-end
NovaSeq X 10B 2 x 50bp Paired-end
NovaSeq X 10B 1 x 200bp Single-end
NovaSeq X 10B 2 x 100bp Paired-end
NovaSeq X 10B 300bp Single-end
NovaSeq X 10B 2 x 150bp Paired-end
NovaSeq X 25B 1 x 100bp Single-end
NovaSeq X 25B 2 x 50bp Paired-end
NovaSeq X 25B 1 x 200bp Single-end
NovaSeq X 25B 2 x 100bp Paired-end
NovaSeq X 25B 300bp Single-end
NovaSeq X 25B 2 x 150bp Paired-end
AVITI sequencing modes
AVITI Cloudbreak High 2x300
AVITI Cloudbreak Medium 2x300
AVITI Cloudbreak High 2x150
AVITI Cloudbreak Medium 2x150
AVITI Cloudbreak High 2x75
AVITI Cloudbreak Medium 2x75
AVITI Cloudbreak Low 2x150
PacBio sequencing modes
PacBio Long Reads
ONT sequencing modes
Third generation sequencing
Number of sequencing runs
How many times do you want to sequence the same sample?
Custom primer
Does the sequencer need a custom primer to cluster your library?
Forward read
Custom primer name
Reverse read
Custom primer name
Index1 (i7)
Custom primer name
Index2 (i5)
Custom primer name
PhiX spike-in
PhiX is a control library that helps with sequencing quality of amplicons and low diversity libraries.
PhiX spike-in percentage
Please choose a percentage of PhiX to be spiked into the final pool.
Common Sample Information
User prepared library
Is the sample a ready to load library?
Pool Concentration (ng/μL)
Concentration of the prepared library pool (up to 3 decimal places)
Pool Name / Tube Label
Pool name should be the same as is written on the tube that you will send
Sample type
----Please select a sample type----
RNA_total
RNA_small
RNA_low amount
rRNA depleted RNA
mRNA
g_DNA
PCR Fragments
BAC_DNA
IP_DNA purified
WaferGen/DropSeq Library
Single-cell half-plate (48 samples, 1.-6. column)
N.A.
Application
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De Novo-Seq
Reseq
ChIP-Seq
Methylation-Seq
targeted DNA methylation analysis
totalRNA-Seq
Small RNA-Seq / miRNA-Seq
stranded mRNA-Seq
stranded rRNAminus RNA-Seq
Low input mRNA-Seq
Strand-Seq
ExomeCapture-Seq
CustomCapture-Seq
AmpliSeq
ATAC-Seq
3Prime-end Quant-Seq Forward
3Prime-end Quant-Seq Reverse
Quant-Seq FWD-UMI
10X genomics v2 (26bp)
10X genomics v3 (28bp)
10X scATAC-Seq
scRNA-Seq / SMART-Seq
scATAC-seq
Multiome-scATAC-Seq
BD-Rhapsody Ab-Seq
CUT & RUN
CUT & Tag
SELEX
CLIP-Seq
Hi-C
Tn5-gDNA-seq
gRNA-20 bases
circRna
RAD-seq
third generation sequencing
Average fragment size
Multiplexing Information
Number of samples (or samples in the pool)
Total number of samples you plan to submit. In case you are submitting a pool of multiplexed samples, please enter the total number of samples in the pool. As well as instructions on what index types were used for each sample.
Do we need to pool the samples?
yes
no
If you are submitting multiple samples, please indicate whether they need to be pooled. If you want to have specific pooling scheme, please submit each pool as a separate order.
Should we demultiplex the samples?
yes
no
Is the library already Multiplexed?
yes
no
What type of index?
None
Single Index
Dual Index
Barcode Length
4
6
8
10
12
16
UMI (Unique Molecular Identifier)
Does your library contain UMIs that need to be processed?
Forward read
UMI description (e.g., '8bp at read start', 'positions 1-10')
Reverse read
UMI description (e.g., '8bp at read start', 'positions 1-10')
Index1 (i7)
UMI description (e.g., '8bp at read start', 'positions 1-10')
Index2 (i5)
UMI description (e.g., '8bp at read start', 'positions 1-10')
Individual Sample Information
Post-Processing Information
Alignment required
yes
no
Reference genome
*
Other
E. coli [Legacy]
phix174 (PhiX 174)
calJac3 (Marmoset)
GRCh37/hg19 (Human)
ce6 (C. elegans)
oryLat2 (Medaka)
panTro3 (Chimpanzee)
ponAbe2 (Orangutan)
rheMac2 (Rhesus)
rn4 (Rat)
borPer (Bordetella pertusis)
S. pombe
tBrucei14032012 (T. brucei)
bosTau7 (Cow)
droVir (D.virilis)
sacCer3 (Yeast)
mm10 (Mouse)
susScr3(Pig)
mg1655 (e. coli)
galGal4 (Chicken)
tair10 (Arabidopsis thaliana)
vibCho (Vibrio cholerae O1 Biovar N16961 El Tor)
bsubtilis (Bacillus subtilis )
acinbaumniph24 (Acinetobacter)
neisseriaMeningitidis
Neisseria meningitidis serogroup B strain MC58 genome
GRCh38/hg38 (Human)
dm6 (D. melanogaster)
danRer11 (Zebrafish)
streptococcus_pyogenes
streptococcus_pneumonia
petMar3 (Petromyzon marinus)
psePut (Pseudomonas putida)
GRCm39 (Mus musculus)
mpmv (Mason-Pfizer monkey virus)
Input the host species name
Remarks
Please enter any additional information and specifics related to the sequencing of your samples and downstream analyses.
Have you performed QC of the pool via Bioanalyzer or Tapestation?
*
yes
no
Click the button below to download a submission file. Send us this file, along with a pdf/image of the bioanlayzer/Tapestation trace of your library/sample.