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EMBL Genomics Core Facility
Sample Submission
User Information
Username
*
Email
*
Group Name
*
Sequencer Settings
Sequencing Technology
*
Illumina
Oxford Nanopore Technologies
PacBio
Illumina sequencing modes
MiSeq i100 5M 1 x 300bp (Single-end)
MiSeq i100 5M 2 x 150bp (Paired-end)
MiSeq i100 5M 1 x 600bp (Single-end)
MiSeq i100 5M 2 x 300bp (Paired-end)
MiSeq i100 25M 1 x 100bp (Single-end)
MiSeq i100 25M 2 x 50bp (Paired-end)
MiSeq i100 25M 1 x 300bp (Single-end)
MiSeq i100 25M 2 x 150bp (Paired-end)
MiSeq i100 25M 1 x 600bp (Single-end)
MiSeq i100 25M 2 x 300bp (Paired-end)
MiSeq i100 25M 2 x 500bp (Paired-end)
NextSeq2000 P1 1 x 100bp (Single-end)
NextSeq2000 P1 2 x 50bp (Paired-end)
NextSeq2000 P1 1 x 300bp (Single-end)
NextSeq2000 P1 2 x 150bp (Paired-end)
NextSeq2000 P1 1 x 600bp (Single-end)
NextSeq2000 P1 2 x 300bp (Paired-end)
NextSeq2000 P2 1 x 100bp (Single-end)
NextSeq2000 P2 2 x 50bp (Paired-end)
NextSeq2000 P2 1 x 200bp (Single-end)
NextSeq2000 P2 2 x 100bp (Paired-end)
NextSeq2000 P2 1 x 300bp (Single-end)
NextSeq2000 P2 2 x 150bp (Paired-end)
NextSeq2000 P2 1 x 600bp (Single-end / 300 M clusters!)
NextSeq2000 P2 2 x 300bp (Paired-end / 300 M clusters!)
NextSeq2000 P4 1 x 50bp (Single-end)
NextSeq2000 P4 2 x 36bp (Paired-end)
NextSeq2000 P4 1 x 100bp (Single-end)
NextSeq2000 P4 2 x 50bp (Paired-end)
NovaSeq X 1.5B 1 x 100bp (Single-end)
NovaSeq X 1.5B 2 x 50bp (Paired-end)
NovaSeq X 1.5B 1 x 200bp (Single-end)
NovaSeq X 1.5B 2 x 100bp (Paired-end)
NovaSeq X 1.5B 1 x 300bp (Single-end)
NovaSeq X 1.5B 2 x 150bp (Paired-end)
NovaSeq X 5B 1 x 100bp (Single-end)
NovaSeq X 5B 2 x 50bp (Paired-end)
NovaSeq X 5B 1 x 200bp (Single-end)
NovaSeq X 5B 2 x 100bp (Paired-end)
NovaSeq X 5B 1 x 300bp (Single-end)
NovaSeq X 5B 2 x 150bp (Paired-end)
NovaSeq X 10B 1 x 100bp (Single-end)
NovaSeq X 10B 2 x 50bp (Paired-end)
NovaSeq X 10B 1 x 200bp (Single-end)
NovaSeq X 10B 2 x 100bp (Paired-end)
NovaSeq X 10B 1 x 300bp (Single-end)
NovaSeq X 10B 2 x 150bp (Paired-end)
NovaSeq X 25B 1 x 100bp (Single-end)
NovaSeq X 25B 2 x 50bp (Paired-end)
NovaSeq X 25B 1 x 200bp (Single-end)
NovaSeq X 25B 2 x 100bp (Paired-end)
NovaSeq X 25B 1 x 300bp (Single-end)
NovaSeq X 25B 2 x 150bp (Paired-end)
AVITI sequencing modes
PacBio sequencing modes
PacBio Long Reads
ONT sequencing modes
ONT GridION
ONT PromethION
Number of sequencing runs
How many times do you want to sequence the same sample?
Custom primer
Does the sequencer need a custom primer to cluster your library?
Forward read
Custom primer name
Reverse read
Custom primer name
Index1 (i7)
Custom primer name
Index2 (i5)
Custom primer name
PhiX spike-in
PhiX is a control library that helps with sequencing quality of amplicons and low diversity libraries.
PhiX spike-in percentage
Please choose a percentage of PhiX to be spiked into the final pool.
Common Sample Information
User prepared library
Is the sample a ready to load library?
Pool Concentration (ng/μL)
Concentration of the prepared library pool (use either '.' or ',' as decimal separator)
Pool Name / Tube Label
Pool name should be the same as is written on the tube that you will send. Please do not use the following characters: /, \, :, ;, *, ?, <, >, |,
Sample type
----Please select a sample type----
BAC_DNA
g_DNA
IP_DNA purified
mRNA
N.A.
PCR Fragments
Plasma (Olink only)
RNA_low amount
RNA_small
RNA_total
rRNA depleted RNA
Serum (Olink only)
Single-cell half-plate (48 samples, 1.-6. column)
WaferGen/DropSeq Library
Application
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10X genomics v2 (26bp)
10X genomics v3 - v4 (28bp)
10X scATAC-Seq
3Prime-end Quant-Seq Forward
3Prime-end Quant-Seq Reverse
AmpliSeq
ATAC-Seq
Aviti24 Teton
BD-Rhapsody Ab-Seq
ChIP-Seq
circRna
CLIP-Seq
CustomCapture-Seq
CUT & RUN
CUT & Tag
De Novo-Seq
Direct RNA ONT sequencing
ExomeCapture-Seq
gRNA-20 bases
Hi-C
Low input mRNA-Seq
Methylation-Seq
mRNA-Seq long insert
Multiome-scATAC-Seq
Olink
Quant-Seq FWD-UMI
RAD-seq
Reseq
scATAC-seq
scRNA-Seq / SMART-Seq
SELEX
Small RNA-Seq / miRNA-Seq
Strand-Seq
stranded mRNA-Seq
stranded rRNAminus RNA-Seq
targeted DNA methylation analysis
third generation sequencing
Tn5-gDNA-seq
totalRNA-Seq
Average fragment size
Multiplexing Information
Number of samples (or samples in the pool)
Total number of samples you plan to submit. In case you are submitting a pool of multiplexed samples, please enter the total number of samples in the pool. As well as instructions on what index types were used for each sample.
Should we pool your samples equimolarly?
yes
no
Choose No to provide an individual pooling ratio for every sample. Ratios can be entered as numeric weights (for example 1, 1, 2) or as percentages that total 100%.
Should we demultiplex the samples?
yes
no
Is the library already Multiplexed?
yes
no
Do you also want the raw data from the sequencer?
yes
no
Raw data consists of the sequencer's image files (for example BCL) or electrical-signal files (for example POD5).
What type of index?
None
Single Index
Dual Index
Barcode Length
4
6
8
10
12
16
UMI (Unique Molecular Identifier)
Does your library contain UMIs that need to be processed?
Forward read
UMI description (e.g., '8bp at read start', 'positions 1-10')
Reverse read
UMI description (e.g., '8bp at read start', 'positions 1-10')
Index1 (i7)
UMI description (e.g., '8bp at read start', 'positions 1-10')
Index2 (i5)
UMI description (e.g., '8bp at read start', 'positions 1-10')
Individual Sample Information
Oxford Nanopore Technologies (ONT) Barcode Information:
Please provide barcode names using the nomenclature
"barcodeXX"
(e.g., "barcode01", "barcode02", etc.) in the Index 1 field.
Post-Processing Information
Alignment required
yes
no
Reference genome
acinbaumniph24 (Acinetobacter)
borPer (Bordetella pertusis)
bosTau7 (Cow)
bsubtilis (Bacillus subtilis )
calJac3 (Marmoset)
ce6 (C. elegans)
danRer11 (Zebrafish)
dm6 (D. melanogaster)
droVir (D.virilis)
E. coli [Legacy]
galGal4 (Chicken)
GRCh37/hg19 (Human)
GRCh38/hg38 (Human)
GRCm39 (Mus musculus)
mg1655 (e. coli)
mm10 (Mouse)
mpmv (Mason-Pfizer monkey virus)
Neisseria meningitidis serogroup B strain MC58 genome
neisseriaMeningitidis
oryLat2 (Medaka)
Other
panTro3 (Chimpanzee)
petMar3 (Petromyzon marinus)
phix174 (PhiX 174)
ponAbe2 (Orangutan)
psePut (Pseudomonas putida)
rheMac2 (Rhesus)
rn4 (Rat)
S. pombe
sacCer3 (Yeast)
streptococcus_pneumonia
streptococcus_pyogenes
susScr3(Pig)
tair10 (Arabidopsis thaliana)
tBrucei14032012 (T. brucei)
vibCho (Vibrio cholerae O1 Biovar N16961 El Tor)
Input the host species name
Remarks
Please enter any additional information and specifics related to the sequencing of your samples and downstream analyses.
Have you performed QC of the pool via Bioanalyzer or Tapestation?
*
yes
no
Click the button below to submit your registration.